recombinant mouse scf miltenyi (Miltenyi Biotec)

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Miltenyi Biotec recombinant mouse scf miltenyi
Recombinant Mouse Scf Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+scf/Mouse+SCF%2C+premium+grade/10__1016_slash_j__celrep__2026__117140-314-76-79
Average 93 stars, based on 9 article reviews

recombinant mouse scf miltenyi - by Bioz Stars, 2026-11

93/100 stars

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shRNA:

Article Title: miR-196b target screen reveals mechanisms maintaining leukemia stemness with therapeutic potential
Article Snippet: .. To determine the size of each shRNA pool to obtain sufficient representation of each shRNA hairpin from the resultant leukemias in the in vivo RNAi screen, murine MLL-AF9 leukemic splenocytes were prestimulated in growth medium containing RPMI 1640 supplemented with 20% FBS (Hyclone), 60 ng/ml mouse SCF, 20 ng/ml mouse IL6, and 20 ng/ml mouse IL3 (Miltenyi Biotech). .. 15 × 10 6 MLL-AF9 cells were transduced in growth medium plus 10 μg/ml polybrene with a lentiviral pool containing over 199,000 unique barcodes (Broad Institute of MIT Harvard) at an MOI of ∼0.1–0.2.

In Vivo:

Recombinant:

Article Title: Type I interferon limits mast cell–mediated anaphylaxis by controlling secretory granule homeostasis
Article Snippet: .. TNP-conjugated BSA (LGC Biosearch Technologies, Middlesex, UK, T5050-10) and 4-methylumbellyferyl-N-acetyl-β-glucosaminide (4-MUAG) (Sigma, St. Louis, MO, M2133), recombinant mouse IL-3 and mouse SCF (Peprotech, Rockey Hill, NJ, 213–13 and 250–03, respectively), recombinant mouse Thrombopoietin (Wako Pure Chemicals, Osaka, Japan, 202–19611), recombinant mouse IFN-α (Miltenyi Biotec 130-093-131), and the STAT3 inhibitor S3I-201 (Merck Millipore, Burlington, MA; hereafter MM, 573102) were purchased. ..

Article Title: A common signaling pathway leading to degranulation in mast cells and its regulation by CCR1-ligand.
Article Snippet: .. Mouse SCF was obtained from Miltenyi Biotec Gmbh (Bergisch, Gladbach) and recombinant mouse HSP70-A1 (low endotoxin) was from Enzo Life Sciences (Farmingdale, NY). .. Primary polyclonal rabbit antibodies used for immunoblotting were those against phosphorylated form of PI3K, Ras, Raf, PLCγ1 (Tyr783), Akt (Ser473), ERK1/2, p38, JNK IKK or IKK, and those against total Akt, ERK1/2, p38 or JNK, all of which were from Cell Signaling Technology (Danvers, MA).

Cell Culture:

Article Title: Oncogenic IL7R gain-of-function mutations in childhood T-cell acute lymphoblastic leukemia
Article Snippet: .. Bone marrow cells were harvested from tibia and femur of Il7r −/− or Il2rg −/− mice, and progenitors were enriched by lineage cell depletion kit (Miltenyi Biotec) and cultured in X-vivo 10 medium (Bio Whittaker) supplemented with 5% FBS, mouse SCF (100 ng/ml), mouse IL-6 (50 ng/ml) and flt-3 ligand (100 ng/ml) (PeproTech). .. After 48 h, cells were infected on RetroNectin (TaKaRa)-coated plates overnight with different retroviral supernatant from the packaging line, and the infection was repeated after 72 h. On the fourth day, cells were harvested, washed and cultured with or without IL-7.

Blocking Assay:

Article Title: Cyba -deficient mice display an increase in hematopoietic stem cells and an overproduction of immunoglobulins
Article Snippet: Antibodies for the detection of murine CD135 (Flt3), CD34, CD16/32, and CD127 (IL-7R±) were from eBioscience, Barcelona, Spain. .. Mouse IL-3, mouse SCF, mouse TPO, mouse Flt3-Ligand, the Lineage Cell Depletion Kit for mice, FcR Blocking reagent and murine flow cytometry antibodies (CD3e, CD4, CD8a, CD11b, CD19, CD43, CD45R (B220), CD117 (c-Kit), anti-Gr1, anti-Sca-1, anti-Ter-119, anti-IgM) were from Miltenyi Biotec, Madrid, Spain. .. SuperScript II reverse transcriptase and RNase OUT ribonuclease inhibitor were from Invitrogen, Thermo Fisher Scientific (Madrid, Spain).

Flow Cytometry:

other:

Article Title: An Id2 RFP -Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials
Article Snippet: Mouse SCF, premium grade , Miltenyi Biotec , Cat# 130-101-693.

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